Conformation and structural fluctuations of a 218 nucleotides long rRNA fragment: 4-thiouridine as an intrinsic photolabelling probe

Abstract

The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity electrophoresis system based on… (More)
DOI: 10.1093/nar/19.13.3653

Topics

Cite this paper

@article{Dubreuil1991ConformationAS, title={Conformation and structural fluctuations of a 218 nucleotides long rRNA fragment: 4-thiouridine as an intrinsic photolabelling probe}, author={Y. L. Dubreuil and A. Expert-Bezancon and A. Favre}, journal={Nucleic acids research}, year={1991}, volume={19 13}, pages={3653-60} }