Confocal ratio-imaging of intracellular pH in unfertilised mouse oocytes.

@article{House1994ConfocalRO,
  title={Confocal ratio-imaging of intracellular pH in unfertilised mouse oocytes.},
  author={Courtenay House},
  journal={Zygote},
  year={1994},
  volume={2 1},
  pages={37-45}
}
Unfertilised mouse oocytes absorbed the pH-sensitive fluoroprobe SNARF-1-AM (carboxyseminaph-thorhodafluor-1-acetoxymethylester), the ester being hydrolysed by an intracellular esterase. Ratio-imaging of oocytes containing the resultant SNARF-1 excited by laser light (514 nm) has been obtained by scanning confocal microscopy with appropriate barrier filters to monitor emission maxima about 590 and 640 nm recorded simultaneously in separate channels of the framestore. Images produced by pixel-by… CONTINUE READING