Confocal microscopy study of uptake kinetics of alpha-lactalbumin and beta-lactoglobulin onto the cation-exchanger SP Sepharose FF.

@article{ElSayed2009ConfocalMS,
  title={Confocal microscopy study of uptake kinetics of alpha-lactalbumin and beta-lactoglobulin onto the cation-exchanger SP Sepharose FF.},
  author={Mayyada M. H. El-Sayed and Howard A. Chase},
  journal={Journal of separation science},
  year={2009},
  volume={32 18},
  pages={3246-56}
}
Confocal laser scanning microscopy (CLSM) was used to study single- and two-component protein uptake for alpha-lactalbumin (ALA) and beta-lactoglobulin (BLG), as models for whey proteins, to SP Sepharose FF at pH 3.7 during batch experiments in a finite bath. By coupling a fluorescent dye with the protein molecule, the penetration into individual adsorbent particles at different times during batch uptake was visualised. In a single-component system, BLG penetrated fast into the adsorbent beads… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…