Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids.

@article{Apellniz2010ConfocalMO,
  title={Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids.},
  author={Beatriz Apell{\'a}niz and Ana J. Garc{\'i}a-S{\'a}ez and Nerea Huarte and Renate E Kunert and Karola Vorauer-Uhl and Hermann Katinger and Petra Schwille and Jos{\'e} Luis Nieva},
  journal={FEBS letters},
  year={2010},
  volume={584 8},
  pages={1591-6}
}
The broadly neutralizing anti-HIV-1 2F5 monoclonal antibody recognizes a gp41 epitope proximal to the viral membrane. Potential phospholipid autoreactivity at cell surfaces has raised concerns about the use of this antibody for development of vaccines or immunotherapy. In this study, confocal microscopy of giant unilamellar vesicles (GUVs) was used to assess 2F5 reactivity with phospholipids assembled into bilayers with surface charge and curvature stress approximating those of the eukaryotic… CONTINUE READING

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