Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.

@article{FischerParton2000ConfocalMO,
  title={Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.},
  author={Sabine Fischer-Parton and Richard M. Parton and Patrick C Hickey and Jan Dijksterhuis and Helen A Atkinson and Nick D Read},
  journal={Journal of microscopy},
  year={2000},
  volume={198 Pt 3},
  pages={246-59}
}
Confocal microscopy of amphiphilic styryl dyes has been used to investigate endocytosis and vesicle trafficking in living fungal hyphae. Hyphae were treated with FM4-64, FM1-43 or TMA-DPH, three of the most commonly used membrane-selective dyes reported as markers of endocytosis. All three dyes were rapidly internalized within hyphae. FM4-64 was found best for imaging the dynamic changes in size, morphology and position of the apical vesicle cluster within growing hyphal tips because of its… CONTINUE READING
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