Confocal microscopy of FM4‐64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae

@article{FischerParton2000ConfocalMO,
  title={Confocal microscopy of FM4‐64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae},
  author={Sabine Fischer-Parton and Richard M. Parton and Patrick C. Hickey and Jan Dijksterhuis and Helen A. Atkinson and Nick D Read},
  journal={Journal of Microscopy},
  year={2000},
  volume={198}
}
Confocal microscopy of amphiphilic styryl dyes has been used to investigate endocytosis and vesicle trafficking in living fungal hyphae. Hyphae were treated with FM4‐64, FM1‐43 or TMA‐DPH, three of the most commonly used membrane‐selective dyes reported as markers of endocytosis. All three dyes were rapidly internalized within hyphae. FM4‐64 was found best for imaging the dynamic changes in size, morphology and position of the apical vesicle cluster within growing hyphal tips because of its… 

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