Confocal microscopy of F-actin distribution in Xenopus oocytes.

  title={Confocal microscopy of F-actin distribution in Xenopus oocytes.},
  author={Amy D Roeder and David L. Gard},
  volume={2 2},
We have used rhodamine-conjugated phalloidin and confocal microscopy to examine the organisation of filamentous actin (F-actin) during oogenesis in Xenopus laevis. F-actin was restricted to a thin shell in the cortex of oogonia and post-mitotic oocytes less than 35 microns in diameter. In oocytes with diameters of 35-50 microns, F-actin was observed in three cellular domains: in the cortex, in the germinal vesicle (GV) and in a network of cytoplasmic cables. Initially, actin cables were… CONTINUE READING


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