Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes.

@article{Dekken1990ConfocalMA,
  title={Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes.},
  author={Herman van Dekken and A van Rotterdam and Richard R Jonker and H T M Van der Voort and G. J. Brakenhoff and Jan G. J. Bauman},
  journal={Journal of microscopy},
  year={1990},
  volume={158 Pt 2},
  pages={207-14}
}
A scanning confocal microscope was used to investigate the spatial positions of specific regions within blood cell nuclei. These centromeric regions were fluorescently labelled by in-situ hybridization to suspended nuclei with a centromere-1-specific DNA probe. The 3-D image data sets, obtained by optical sectioning of the cells, were used to determine the spatial position of the centromeric regions in the nuclei by means of specially developed software. The centromeres were found to be… CONTINUE READING