Confocal microscopy and albumin penetration into contact lenses.

  title={Confocal microscopy and albumin penetration into contact lenses.},
  author={Doerte Luensmann and Mary-Ann Glasier and Feng Zhang and Vladimir Bantseev and Trefford Simpson and Lyndon A Jones},
  journal={Optometry and vision science : official publication of the American Academy of Optometry},
  volume={84 9},
PURPOSE To develop a novel in vitro method to detect the depth of penetration of the tear film protein albumin into contact lens materials using confocal laser scanning microscopy (CLSM). METHODS A poly-HEMA-based hydrogel (etafilcon A) and a silicone hydrogel material (lotrafilcon B) were examined. In vitro, bovine serum albumin (BSA) was labeled with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein hydrochloride (DTAF). The lenses were incubated in this protein solution (0.5 mg/ml) at 37… CONTINUE READING