Confocal laser scanning microscopy of apoptosis in organogenesis-stage mouse embryos.

@article{Zucker1998ConfocalLS,
  title={Confocal laser scanning microscopy of apoptosis in organogenesis-stage mouse embryos.},
  author={Robert Martin Zucker and Susan Allie Hunter and Jason M. Rogers},
  journal={Cytometry},
  year={1998},
  volume={33 3},
  pages={
          348-54
        }
}
Confocal laser scanning microscopy combined with a vital stain has been used to study apoptosis in organogenesis-stage mouse embryos. In order to achieve optical sectioning through embryos, it was necessary to use low power objectives and to prepare the sample appropriately. Mouse embryos were harvested on gestation day 8 or 9 and stained with the vital lysosomal dye, LysoTracker Red. Following incubation in the stain, embryos were fixed in 2% paraformaldehyde overnight, dehydrated in a graded… CONTINUE READING

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