Confocal laser scanning microscopy as an analytical tool in chromatographic research.

@article{Hubbuch2008ConfocalLS,
  title={Confocal laser scanning microscopy as an analytical tool in chromatographic research.},
  author={J{\"u}rgen J Hubbuch and Maria Regina Kula},
  journal={Bioprocess and biosystems engineering},
  year={2008},
  volume={31 3},
  pages={241-59}
}
In recent years, confocal laser scanning microscopy has been developed into a non-invasive tool to probe intra-particle profiles of protein in chromatographic adsorbents. A necessary prerequisite when using this technique lies in the labeling of proteins with fluorescent probes. The quality of the obtained results is thus strongly dependent on the probes used, its sensitivity on experimental parameters and the change of protein characteristics upon binding. In this review, the fundamental… CONTINUE READING