Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS

  title={Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS},
  author={Carlo Manno and Lourdes Figueroa and Robert H Fitts and Eduardo R{\'i}os},
  booktitle={The Journal of general physiology},
Imaging, optical mapping, and optical multisite recording of transmembrane potential (V(m)) are essential for studying excitable cells and systems. The naphthylstyryl voltage-sensitive dyes, including di-8-ANEPPS, shift both their fluorescence excitation and emission spectra upon changes in V(m). Accordingly, they have been used for monitoring V(m) in nonratioing and both emission and excitation ratioing modes. Their changes in fluorescence are usually much less than 10% per 100 mV… CONTINUE READING


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