Confocal imaging of biofilm formation process using fluoroprobed Escherichia coli and fluoro-stained exopolysaccharide.

@article{Maeyama2004ConfocalIO,
  title={Confocal imaging of biofilm formation process using fluoroprobed Escherichia coli and fluoro-stained exopolysaccharide.},
  author={Ryo Maeyama and Yoshimitsu Mizunoe and James M Anderson and Masao Tanaka and Takehisa Matsuda},
  journal={Journal of biomedical materials research. Part A},
  year={2004},
  volume={70 2},
  pages={274-82}
}
We developed a novel method of evaluating biofilm architecture on a synthetic material using green fluorescent protein-expressing Escherichia coli and red fluorescence staining of exopolysaccharides. Confocal laser scanning microscopy observation revealed the time course of the change in the in situ three-dimensional structural features of biofilm on a polyurethane film without structural destruction: initially adhered cells are grown to form cellular aggregates and secrete exopolysaccharides… CONTINUE READING

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