Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa.

Abstract

Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by… (More)
DOI: 10.1039/b927012d

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