Confirmation of the helical structure of the 5'/3' termini of the essential DNA packaging pRNA of phage phi 29.

@article{Zhang1995ConfirmationOT,
  title={Confirmation of the helical structure of the 5'/3' termini of the essential DNA packaging pRNA of phage phi 29.},
  author={Chuan Zhang and Timothy L. Tellinghuisen and Peixuan Guo},
  journal={RNA},
  year={1995},
  volume={1 10},
  pages={1041-50}
}
Bacteriophage phi 29 is typical of double-stranded DNA viruses in that its genome is packaged into a preformed procapsid during viral assembly. An intriguing feature of phi 29 is the presence of a 120-base virus-encoded RNA (pRNA) that is indispensable for DNA packaging. Phylogenetic comparison of similar RNAs in numerous phages has revealed that the secondary structure of the pRNA is well conserved. Computer analysis predicts the presence of an extensive segment of helix with three single-base… CONTINUE READING