Confirmation of psaA in all 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis.

@article{Morrison2000ConfirmationOP,
  title={Confirmation of psaA in all 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis.},
  author={K E Morrison and Duncan Lake and Jeremy Crook and George M. Carlone and Edwin P Ades and Richard R. Facklam and Jacquelyn S. Sampson},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 1},
  pages={434-7}
}
The gene encoding the pneumococcal surface adhesin A (PsaA) protein, psaA, was confirmed in all Streptococcus pneumoniae serotypes by a newly developed PCR (psaA PCR) assay. Eighty-nine of the 90 serotypes amplified produced an 838-bp fragment; the exception was a serotype 16F strain acquired from the American Type Culture Collection (ATCC). Analysis of 20 additional 16F strains from the United States and Brazil showed that the gene was amplified in all 16F strains, implying that the serotype… CONTINUE READING
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Comparison of a pneumococcal common protein (PsaA) antibody ELISA and a PsaA immune complex ELISA for detection of pneumococcal serum antibody

  • J. A. Tharpe, H. Russell, +5 authors J. S. Sampson
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