Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis.

@article{Mathers2000ConfirmationOC,
  title={Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis.},
  author={William D Mathers and Shannon E Nelson and Judith Lane and Michelle E Wilson and Richard Cutler Allen and Robert Folberg},
  journal={Archives of ophthalmology},
  year={2000},
  volume={118 2},
  pages={
          178-83
        }
}
BACKGROUND Acanthamoeba keratitis has commonly been identified with in vivo confocal microscopy and confirmed with histologic examination of an epithelial biopsy specimen. OBJECTIVE To determine if Acanthamoeba keratitis can be verified using polymerase chain reaction (PCR) of epithelial biopsy specimens. METHODS Epithelial specimens from patients with suspected Acanthamoeba keratitis by confocal microscopy were tested for Acanthamoeba with PCR of Acanthamoeba ribosomal DNA. RESULTS… CONTINUE READING

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