Confining Brownian motion of single nanoparticles in an ABELtrap

  title={Confining Brownian motion of single nanoparticles in an ABELtrap},
  author={Maria Dienerowitz and Thomas Heitkamp and Thomas Gottschall and Jens Limpert and Michael B{\"o}rsch},
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1–7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of… 
Measuring nanoparticle diffusion in an ABELtrap
Monitoring the Brownian motion of individual nanoscopic objects is key to investigate their transport properties and interactions with their close environment. Most techniques rely on transient
Single-molecule FRET dynamics of molecular motors in an ABEL Trap
The capabilities of the ABEL trap are exemplified in performing extended timescale smFRET measurements on the molecular motor Rep, which is crucial for removing protein blocks ahead of the advancing DNA replication machinery and for restarting stalled DNA replication.
A Handy Liquid Metal Based Non-Invasive Electrophoretic Particle Microtrap
A handy liquid metal based non-invasive particle microtrap is proposed and demonstrated and can be easily designed and fabricated at any location of a microfluidic chip to perform precise particle trapping and releasing without disturbing the microchannel itself.
Fast ATP-dependent Subunit Rotation in Reconstituted FoF1-ATP Synthase Trapped in Solution
It is reported that kinetic monitoring of functional rotation can be prolonged from milliseconds to seconds by utilizing an Anti-Brownian electrokinetic trap (ABEL trap), and broad distributions of ATP-dependent catalytic rates were revealed.
Observing monomer: dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap
The oligomerization state of the human NTSR1 tagged with mRuby3 is reported by dissolving the plasma membranes of living HEK293T cells into 10 nm-sized soluble lipid nanoparticles by addition of styrene-maleic acid copolymers (SMALPs).
Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase
The lifetimes of fluorescence donor and acceptor dyes are analyzed to distinguish between smFRET signals of conformational changes and potential artefacts to prevent wasteful ATP hydrolysis.
Structural Asymmetry and Kinetic Limping of Single Rotary F-ATP Synthases
The enzyme’s rotary progression during ATP hydrolysis is monitored by three single-molecule techniques: fluorescence video-microscopy with attached actin filaments, Förster resonance energy transfer between pairs of fluorescence probes, and a polarization assay using gold nanorods, and it is found that one dwell in the three-steppedrotary progression lasting longer than the other two by a factor of up to 1.6.
Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET
G protein-coupled receptor 1 was found to be monomeric in liposomes, with a small fraction being dimeric and oligomeric, showing homoFRET, and agonist binding to NTSR1 was demonstrated by time-resolved single-molecule Förster resonance energy transfer (smFRET), using neurotensin labeled with the fluorophore ATTO594.
The regulatory subunit ε in Escherichia coli FOF1-ATP synthase.


Manipulating freely diffusing single 20-nm particles in an Anti-Brownian Electrokinetic Trap (ABELtrap)
An ABELtrap based on a laser focus pattern generated by a pair of acousto-optical beam deflectors and controlled by a programmable FPGA chip is presented, which increased observation times of a single particle by a factor of 1000.
Electrokinetic trapping at the one nanometer limit
A feedback-based anti-Brownian electrokinetic trap in which classical thermal noise is compensated to the maximal extent allowed by quantum measurement noise is developed, which greatly extends the size range of molecules that can be studied by room temperature feedback trapping, and opens the door to further studies of the binding of unmodified proteins to DNA in free solution.
Observing conformations of single FoF1-ATP synthases in a fast anti-Brownian electrokinetic trap
A version of an ABELtrap with a laser focus pattern generated by electro-optical beam deflectors and controlled by a programmable FPGA is presented, which could hold single fluorescent nanobeads for more than 100 seconds and increase the observation times of a single particle more than 1000-fold.
Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer.
An Anti-Brownian Electrokinetic trap capable of trapping individual fluorescently labeled protein molecules in aqueous buffer is presented, and confinement of single fluorophores of the dye Cy3 in water is shown.
Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap.
This work has developed a device called the anti-Brownian electrokinetic (ABEL) trap to significantly prolong the observation time of single biomolecules in solution and applied the ABEL trap method to explore the photodynamics and enzymatic properties of a variety of biomolecule in aqueous solution.
Method for trapping and manipulating nanoscale objects in solution
We present a device that allows a user to trap a single nanoscale object in solution at ambient temperature, and then to position the trapped object with nanoscale resolution. This anti-Brownian
An Adaptive Anti-Brownian ELectrokinetic trap with real-time information on single-molecule diffusivity and mobility.
An adaptive Anti-Brownian ELectrokinetic (ABEL) trap capable of extracting estimates of the diffusion coefficient and mobility of single trapped fluorescent nanoscale objects such as biomolecules in solution is presented.
Anti-Brownian traps for studies on single molecules.
Control of nanoparticles with arbitrary two-dimensional force fields.
  • A. Cohen
  • Physics
    Physical review letters
  • 2005
An anti-Brownian electrophoretic trap is used to create arbitrary two-dimensional force fields for individual nanoscale objects in solution to study Brownian motion in harmonic, power-law, and double-well potentials.
Watching conformational- and photo-dynamics of single fluorescent proteins in solution
A complex relationship between fluorescence intensity and lifetime that cannot be explained by simple static kinetic models is observed and evidence is obtained for fluctuations in the spontaneous emission lifetime, which is typically assumed to be constant.