Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking.

@article{Daumas2003ConfinedDW,
  title={Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking.},
  author={Fr{\'e}d{\'e}ric Daumas and Nicolas Destainville and Claire Millot and Andr{\'e} Lopez and David Dean and Laurence Salom{\'e}},
  journal={Biophysical journal},
  year={2003},
  volume={84 1},
  pages={356-66}
}
Single particle tracking is a powerful tool for probing the organization and dynamics of the plasma membrane constituents. We used this technique to study the micro -opioid receptor belonging to the large family of the G-protein-coupled receptors involved with other partners in a signal transduction pathway. The specific labeling of the receptor coupled to a T7-tag at its N-terminus, stably expressed in fibroblastic cells, was achieved by colloidal gold coupled to a monoclonal anti T7-tag… CONTINUE READING
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Stochastic Processes in Physics and Chemistry

  • N. G. Van Kampen
  • North-Holland, Amsterdam.
  • 2001
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