Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

@inproceedings{Ji2018ConferringDV,
  title={Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system},
  author={Xiang Ji and Xiaomin Si and Yi Zhang and Huawei Zhang and Feng Zhang and Caixia Gao},
  booktitle={Genome biology},
  year={2018}
}
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off… CONTINUE READING
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Conferring DNA virus resistance with high specificity in plants using virus-inducible genome editing system

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Conferring DNA virus resistance with high specificity in plants using virus-inducible genome editing system https://www.ncbi.nlm.nih.gov/bioproject/PRJNA492959

  • X Ji, X Si, Y Zhang, H Zhang, F Zhang, C. Gao
  • Accessed 24 Sept . Ji et al. Genome Biology
  • 2018

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