• DOI:10.1042/BJ20090785
• Corpus ID: 6106028

Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae.

@article{Fei2009ConditionsOE,
  title={Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae.},
  author={Weihua Fei and Han Wang and Xin Fu and Christopher Bielby and Hongyuan Yang},
  journal={The Biochemical journal},
  year={2009},
  volume={424 1},
  pages={
          61-7
        }
}

• Weihua Fei, Han Wang, +2 authors Hongyuan Yang
• Published 15 November 2009
• Biology
• The Biochemical journal

LDs (lipid droplets) are cellular organelles which can be found in nearly all eukaryotic cells. Despite their importance in cell biology, the mechanism underlying LD biogenesis remains largely unknown. In the present study we report that conditions of ER (endoplasmic reticulum) stress stimulate LD formation in Saccharomyces cerevisiae. We found that LDs accumulated in yeast mutants with compromised protein glycosylation or ER-associated protein degradation. Moreover, tunicamycin and Brefeldin A, agents which induce ER stress, were found to stimulate LD formation. In contrast, the restoration of protein glycosylation reduced LD accumulation. Interestingly, enhanced neutral lipids synthesis and LD formation under conditions of ER stress was not dependent on Ire1p. Lastly, we demonstrated that the absence of LDs did not compromise cell viability under ER stress. Our results suggest that although more LDs are produced, LDs are not essential to cell survival under ER stress.