Conditions leading to secretion of a normally periplasmic protein in Escherichia coli.

@article{Pags1987ConditionsLT,
  title={Conditions leading to secretion of a normally periplasmic protein in Escherichia coli.},
  author={Jean Marie Pag{\`e}s and Jamila Anba and Claude J. Lazdunski},
  journal={Journal of bacteriology},
  year={1987},
  volume={169 4},
  pages={
          1386-90
        }
}
The phosphate-binding protein (PhoS) is a periplasmic protein which is part of the high-affinity phosphate transport system of Escherichia coli. Hyperproduction of PhoS in strains carrying a multicopy plasmid containing phoS led to partial secretion of the protein. By 6 h after transfer to phosphate-limiting medium, about 13% of the total newly synthesized PhoS was secreted to the medium. Kinetic studies demonstrated that this secretion consists of newly synthesized PhoS. This secretion occurs… CONTINUE READING
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