Conditions for the culture of bovine embryonic myogenic cells.

  title={Conditions for the culture of bovine embryonic myogenic cells.},
  author={T. L. Woods and Clifford W. Smith and Michael G. Zeece and Steven Jeffrey Jones},
  journal={Tissue & cell},
  volume={29 2},
The objective of this experiment was to determine the growth characteristics of bovine embryonic muscle cells and to optimize the growth conditions for these cells using commercially-prepared media and sera. In the first study, the growth of muscle cells isolated from the hindlimb was determined by measuring DNA content. The DNA concentration was lowest (P < 0.001) at 24 h post-plating and increased to a maximum at approximately 60 h. The slopes of creatine kinase activity and fusion index… CONTINUE READING