Conditions for protoplast preparation and regeneration by Actinobacillus succinogenes

@inproceedings{Ma2014ConditionsFP,
  title={Conditions for protoplast preparation and regeneration by Actinobacillus succinogenes},
  author={Yao Ma},
  year={2014}
}
The effect of glycine pretreatment, cell age and the concentration, temperature and work time of lysozyme on protoplast formation and regeneration by Actinobacillus succinogenes ATCC 55618 were studied. And the optimal conditions obtained as follow: the culture was cultivated 6 h in broth added with 1.0% glycine, treated by 0.08 mg/mL lysozyme for 40 min at 37 ℃. As a result, the protoplast formation and regeneration were 96.28% and 42.11% respectively. The study of protoplast formation and… CONTINUE READING