Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts


Using ‘Trovita’ orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (∼4×104/ml) and low mannitol… (More)
DOI: 10.1007/BF00040199


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