Conditioned media from mesenchymal stromal cells restore sodium transport and preserve epithelial permeability in an in vitro model of acute alveolar injury.

@article{Goolaerts2014ConditionedMF,
  title={Conditioned media from mesenchymal stromal cells restore sodium transport and preserve epithelial permeability in an in vitro model of acute alveolar injury.},
  author={Arnaud Goolaerts and Nadia Pellan-Randrianarison and J{\'e}r{\^o}me Larghero and Val{\'e}rie Vanneaux and Yurdagul Uzunhan and Thomas Gille and Nicolas Dard and Carole Plan{\`e}s and Michael A. Matthay and Christine Cl{\'e}rici},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={2014},
  volume={306 11},
  pages={
          L975-85
        }
}
Mesenchymal stromal cells (MSCs) or their media (MSC-M) were reported to reverse acute lung injury (ALI)-induced decrease of alveolar fluid clearance. To determine the mechanisms by which MSC-M exert their beneficial effects, an in vitro model of alveolar epithelial injury was created by exposing primary rat alveolar epithelial cells (AECs) to hypoxia (3% O2) plus cytomix, a combination of IL-1β, TNF-α, and IFN-γ. MSC-M were collected from human MSCs exposed for 12 h to either normoxia (MSC-M… 
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