Conditional overexpression of active transforming growth factor beta1 in vivo accelerates metastases of transgenic mammary tumors.

@article{MuraokaCook2004ConditionalOO,
  title={Conditional overexpression of active transforming growth factor beta1 in vivo accelerates metastases of transgenic mammary tumors.},
  author={Rebecca S. Muraoka-Cook and Hirokazu Kurokawa and Yasuhiro Koh and James T. Forbes and L. Renee Roebuck and Mary Helen Barcellos-Hoff and Susan E. Moody and Lewis A. Chodosh and Carlos L. Arteaga},
  journal={Cancer research},
  year={2004},
  volume={64 24},
  pages={9002-11}
}
To address the role of transforming growth factor (TGF) beta in the progression of established tumors while avoiding the confounding inhibitory effects of TGF-beta on early transformation, we generated doxycycline (DOX)-inducible triple transgenic mice in which active TGF-beta1 expression could be conditionally regulated in mouse mammary tumor cells transformed by the polyomavirus middle T antigen. DOX-mediated induction of TGF-beta1 for as little as 2 weeks increased lung metastases >10-fold… CONTINUE READING