Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development.

@article{Shen2014ConditionalKG,
  title={Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development.},
  author={Zhongfu Shen and Xianliang Zhang and Yongping Chai and Zhiwen Zhu and Peishan Yi and Guoxin Feng and Wei Li and Guangshuo Ou},
  journal={Developmental cell},
  year={2014},
  volume={30 5},
  pages={625-36}
}
Conditional gene knockout animals are valuable tools for studying the mechanisms underlying cell and developmental biology. We developed a conditional knockout strategy by spatiotemporally manipulating the expression of an RNA-guided DNA endonuclease, CRISPR-Cas9, in Caenorhabditis elegans somatic cell lineages. We showed that this somatic CRISPR-Cas9 technology provides a quick and efficient approach to generate conditional knockouts in various cell types at different developmental stages… CONTINUE READING
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