Conditional knockdown of hMRS2 results in loss of mitochondrial Mg + uptake and cell death

@inproceedings{Piskacek2009ConditionalKO,
  title={Conditional knockdown of hMRS2 results in loss of mitochondrial Mg + uptake and cell death},
  author={Martin Piskacek and Ludmila Zotova and G{\'a}bor Zsurka and Rudolf J. Schweyen},
  booktitle={Journal of cellular and molecular medicine},
  year={2009}
}
The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) transport, we have established stable cell lines with conditionally expressing shRNAs directed against hMRS2L. The cells expressing shRNA for several generations exhibited lower steady-state levels of free mitochondrial Mg(2+) ([Mg(2+)](m)) and reduced… CONTINUE READING
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The structure of CorA: a Mg(2+)-selective channel.

Current opinion in structural biology • 2006
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