Conditional gene expression by controlling translation with tetracycline-binding aptamers.

@article{Suess2003ConditionalGE,
  title={Conditional gene expression by controlling translation with tetracycline-binding aptamers.},
  author={Beatrix Suess and Shane Hanson and Christian Berens and Barbara Fink and Ren{\'e}e Schroeder and W. Hillen},
  journal={Nucleic acids research},
  year={2003},
  volume={31 7},
  pages={
          1853-8
        }
}
We present a conditional gene expression system in Saccharomyces cerevisiae which exploits direct RNA-metabolite interactions as a mechanism of genetic control. We inserted preselected tetracycline (tc) binding aptamers into the 5'-UTR of a GFP encoding mRNA. While aptamer insertion generally reduces GFP expression, one group of aptamers displayed an additional, up to 6-fold, decrease in fluorescence upon tc addition. Regulation is observed for aptamers inserted cap-proximal or near the start… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 74 CITATIONS, ESTIMATED 59% COVERAGE

Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast

  • Nucleic acids research
  • 2007
VIEW 5 EXCERPTS
CITES METHODS & BACKGROUND

Development of RNA aptamers and synthetic riboswitch using Capture-SELEX

VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

2 Developing RNA Tools for Engineered Regulatory Systems

' ERICA.DAVIDSON, Andrew D. Ellington
  • 2006
VIEW 3 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Development of aptamer based targeted reversibly attenuated probes

VIEW 5 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2004
2019

CITATION STATISTICS

  • 4 Highly Influenced Citations

References

Publications referenced by this paper.
SHOWING 1-10 OF 15 REFERENCES

A tetracycline-binding RNA aptamer.

  • Bioorganic & medicinal chemistry
  • 2001
VIEW 8 EXCERPTS

An mRNA structure that controls gene expression by binding FMN.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002