Conditional control of gene function by an invertible gene trap in zebrafish.

@article{Ni2012ConditionalCO,
  title={Conditional control of gene function by an invertible gene trap in zebrafish.},
  author={Terri T. Ni and Jianjun Lu and Meiying Zhu and Lisette A. Maddison and Kelli Lynn Boyd and Lindsey Huskey and Bensheng Ju and Daniel Hesselson and Tao P. Zhong and Patrick S Page-McCaw and Didier Y. R. Stainier and Wenbiao Chen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 38},
  pages={15389-94}
}
Conditional mutations are essential for determining the stage- and tissue-specific functions of genes. Here we achieve conditional mutagenesis in zebrafish using FT1, a gene-trap cassette that can be stably inverted by both Cre and Flp recombinases. We demonstrate that intronic insertions in the gene-trapping orientation severely disrupt the expression of the host gene, whereas intronic insertions in the neutral orientation do not significantly affect host gene expression. Cre- and Flp-mediated… CONTINUE READING
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