Concurrent measurements of the free cytosolic concentrations of H+ and Na+ ions with fluorescent indicators

@article{Sheldon2004ConcurrentMO,
  title={Concurrent measurements of the free cytosolic concentrations of H+ and Na+ ions with fluorescent indicators},
  author={Claire Sheldon and Y. M. Cheng and J. N. Church},
  journal={Pfl{\"u}gers Archiv},
  year={2004},
  volume={449},
  pages={307-318}
}
We report a method for the concurrent measurement of intracellular [Na+] ([Na+]i) and pH (pHi) in cells co-loaded with SBFI, a Na+-sensitive fluorophore, and either carboxy SNARF-1 or SNARF-5F, H+-sensitive fluorophores. With the optical filters specified, fluorescence emissions from SBFI and either SNARF derivative were sufficiently distinct to allow the… CONTINUE READING