Pseudomonas aeruginosa, an opportunistic pathogen causes community acquired infections and also hospital acquired infection. Metallo beta lactamase (MBL) producing P. aeruginosa are very difficult to exterminate, as a result they cause high morbidity and mortality among patients infected by them. Strains of P. aeruginosa exhibiting biofilm forming characteristic have been found to resist different antibiotic therapy and also their ability of collagenase formation showed increased pathogenicity. In this study, the difference of collagenase activity and degree of biofilm forming ability was analyzed using both MBL producing and MBL non-producing strains of P. aeruginosa. The study showed that MBL producers have strong Biofilm forming ability and higher collagenase activity and also we found out a correlation existing between these two virulence factors.