Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting.

@article{Sorrell1996ConcordanceOC,
  title={Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting.},
  author={Tania Christine Sorrell and Sharon C. A. Chen and P Ruma and Wieland Meyer and Timothy J. Pfeiffer and David H. Ellis and Alan G. Brownlee},
  journal={Journal of clinical microbiology},
  year={1996},
  volume={34 5},
  pages={1253-60}
}
Sixty-one clinical and forty-nine environmental isolates of Cryptococcus neoformans var. gattii from Australia and the United States were analyzed by random amplification of polymorphic DNA (RAPD), using 12- to 22-mer primers in pairs, and/or PCR fingerprinting with a single primer derived from the microsatellite core sequence of the wild-type phage M13 (5' GAGGGTGGCGGTTCT 3'). Three major genetic profiles were identified by both typing techniques. A single RAPD profile (VGI) predominated among… CONTINUE READING
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