Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings.

@article{VillaDiaz2013ConciseRT,
  title={Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings.},
  author={Luis Gerardo Villa-Diaz and Adam M. Ross and Joerg Lahann and Paul H Krebsbach},
  journal={Stem cells},
  year={2013},
  volume={31 1},
  pages={1-7}
}
Current practices to maintain human pluripotent stem cells (hPSCs), which include induced pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend on the support of feeder cells such as mouse embryonic fibroblasts (MEFs) or an extracellular matrix such as Matrigel. Culture conditions that depend on these undefined support systems limit our ability to interpret mechanistic studies aimed at resolving how hPSCs interact with their extracellular environment to… CONTINUE READING
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