Concise Review: Induced Pluripotency by Defined Factors: Prey of Oxidative Stress

@article{Qi2015ConciseRI,
  title={Concise Review: Induced Pluripotency by Defined Factors: Prey of Oxidative Stress},
  author={Suxia Qi and Zhi Fang and Danli Wang and Pablo Men{\'e}ndez and Ke Yao and Junfeng Ji},
  journal={STEM CELLS},
  year={2015},
  volume={33}
}
Reprogramming somatic cells to pluripotency (induced pluripotent stem cells, iPSCs) via forced expression of defined factors has become one of the most fascinating areas in biomedical research because it holds a tremendous application potential for cell therapy, disease modeling, and drug screening applications. However, cellular reprogramming is a very inefficient and metabolically demanding process commonly associated with genomic instability of the resulting iPSCs. Low reprogramming… 
View on Wiley
ncbi.nlm.nih.gov
13 Citations
Background Citations
6

13 Citations

Citation Type

Citation Type

Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A
TLDR
This study found that a natural compound, vitamin C, augmented pluripotency in iPS cells and reduced unwanted spontaneous differentiation during i PS cells maintenance and establishes a link between vitamin C and epigenetic regulation.
mtDNA Mutagenesis Disrupts Pluripotent Stem Cell Function by Altering Redox Signaling
Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells
TLDR
Clonal reprogramming ruled out the aberrant chromosomes inherited from rare karyotypically abnormal parental cell subpopulation and antioxidant N-acetyl-cysteine added to the culture medium effectively reduced the occurrence of chromosomal aberrations at the early stage of reprograming.
Influence of ATM-Mediated DNA Damage Response on Genomic Variation in Human Induced Pluripotent Stem Cells.
TLDR
It is shown that following reprogramming the early and late replicating genome is differentially affected by CNVs in ATM-deficient iPSCs relative to wild-type i PSCs, and the early replicating regions had increased CNV losses during retroviral reprograming.
Role of metabolism and epigenetics in forced and endogenous neurogenesis in vivo
TLDR
The projects discussed here addressed the implications of epigenetics by investigating the role of Ring1B, the catalytic protein of the Polycomb Repressive Complex 1, and showed that the reprogrammed neurons acquire a deep layer identity, but also that they can develop mature structures and survive until late time points.
Concise Review: Assessing the Genome Integrity of Human Induced Pluripotent Stem Cells: What Quality Control Metrics?
TLDR
It is proposed that the genome of hiPSC lines should be systematically screened at derivation, at least by karyotyping, and then regularly (every 12 weeks) during experiments, for instance with polymerase chain reaction‐based techniques.
Pharmacological Regulation of Oxidative Stress in Stem Cells
TLDR
The defined roles of oxidative Stress in pluripotent stem cells and hematopoietic stem cells are introduced and the potential applications of pharmacological approaches for regulating oxidative stress in regenerative medicine are discussed.
Identification and Successful Negotiation of a Metabolic Checkpoint in Direct Neuronal Reprogramming.
Genome stability of programmed stem cell products
  • U. Martin
  • Biology
    Advanced drug delivery reviews
  • 2017
Unraveling and overcoming hurdles in direct neuronal reprogramming
TLDR
It is demonstrated that a metabolic switch from glycolysis to oxidative phosphorylation is an essential requirement for a successful conversion to occur, as inhibiting the function of the electron transport chain did not improve the process despite the decrease in ROS, but actually entirely blocked the conversion of glia into neurons.
...
1
2
...

References

SHOWING 1-10 OF 72 REFERENCES
The Senescence‐Related Mitochondrial/Oxidative Stress Pathway is Repressed in Human Induced Pluripotent Stem Cells
TLDR
It is demonstrated that somatic mitochondria within human iPSCs revert to an immature ESC‐like state with respect to organelle morphology and distribution, expression of nuclear factors involved in mitochondrial biogenesis, content of mitochondrial DNA, intracellular ATP level, oxidative damage, and lactate generation, and suggest that cellular reprogramming can modulate the mitochondrial/oxidative stress pathway, thus inducing a rejuvenated state capable of escaping cellular senescence.
Epigenetics of Reprogramming to Induced Pluripotency
Assessing the risks of genotoxicity in the therapeutic development of induced pluripotent stem cells.
TLDR
This review will critically discuss current reprogramming technologies particularly in the context of genotoxicity, and the consequences of these alternations for the potential applications of reprogrammed cells.
HIF1α Modulates Cell Fate Reprogramming Through Early Glycolytic Shift and Upregulation of PDK1–3 and PKM2
TLDR
The findings suggest that the early induction of HIF1α targets may be instrumental in iPSC derivation via the activation of a glycolytic program, and implicate the Hif1α pathway as an enabling regulator of cellular reprogramming.
Antioxidant Supplementation Reduces Genomic Aberrations in Human Induced Pluripotent Stem Cells
Senescence impairs successful reprogramming to pluripotent stem cells.
TLDR
Crucially, ablation of different senescence effectors improves the efficiency of reprogramming, suggesting novel strategies for maximizing the generation of iPS cells.
Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state.
Linking the p53 tumor suppressor pathway to somatic cell reprogramming
TLDR
It is shown that reprogramming factors can activate the p53 (also known as Trp53 in mice, TP53 in humans) pathway and silencing of p53 significantly increased the reprograming efficiency of human somatic cells.
A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity
TLDR
Observations indicate that during reprogramming cells increase their intolerance to different types of DNA damage and that p53 is critical in preventing the generation of human and mouse pluripotent cells from suboptimal parental cells.
Vitamin C enhances the generation of mouse and human induced pluripotent stem cells.
...
1
2
3
4
5
...