Concerted interaction between origin recognition complex (ORC), nucleosomes and replication origin DNA ensures stable ORC-origin binding.

@article{Hizume2013ConcertedIB,
  title={Concerted interaction between origin recognition complex (ORC), nucleosomes and replication origin DNA ensures stable ORC-origin binding.},
  author={Kohji Hizume and Masaru Yagura and Hiroyuki Araki},
  journal={Genes to cells : devoted to molecular & cellular mechanisms},
  year={2013},
  volume={18 9},
  pages={764-79}
}
Chromosomal replication origins, where DNA replication is initiated, are determined in eukaryotic cells by specific binding of a six-subunit origin recognition complex (ORC). Many biochemical analyses have showed the detailed properties of the ORC-DNA interaction. However, because of the lack of in vitro analysis, the molecular architecture of the ORC-chromatin interaction is unclear. Recently, mainly from in vivo analyses, a role of chromatin in the ORC-origin interaction has been reported… CONTINUE READING