Concentrative sorting of secretory cargo proteins into COPII-coated vesicles

@article{Malkus2002ConcentrativeSO,
  title={Concentrative sorting of secretory cargo proteins into COPII-coated vesicles},
  author={Per N Malkus and Feng Jiang and Randy Schekman},
  journal={The Journal of Cell Biology},
  year={2002},
  volume={159},
  pages={915 - 921}
}
Here, we show that efficient transport of membrane and secretory proteins from the ER of Saccharomyces cerevisiae requires concentrative and signal-mediated sorting. Three independent markers of bulk flow transport out of the ER indicate that in the absence of an ER export signal, molecules are inefficiently captured into coat protein complex II (COPII)-coated vesicles. A soluble secretory protein, glycosylated pro-alpha-factor (gpalphaf), was enriched approximately 20 fold in these vesicles… CONTINUE READING
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