Concentrations of glycolytic enzymes and other cytosolic proteins in the diffusible fraction of a vertebrate muscle proteome.

@article{Maughan2005ConcentrationsOG,
  title={Concentrations of glycolytic enzymes and other cytosolic proteins in the diffusible fraction of a vertebrate muscle proteome.},
  author={David W. Maughan and Josh A Henkin and Jim O. Vigoreaux},
  journal={Molecular & cellular proteomics : MCP},
  year={2005},
  volume={4 10},
  pages={1541-9}
}
We used a novel microvolumetric technique based on protein diffusion to characterize the subproteome of muscle that consists of diffusible proteins, including those involved in cell metabolism. Muscle fiber segments were mechanically demembranated under mineral oil and transferred into drops of relaxing solution. After the fiber segment was depleted of diffusible proteins, the content of each drop and residual segment was analyzed by one-dimensional polyacrylamide gel electrophoresis. Proteins… CONTINUE READING

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