Concentration and physicochemical characterisation of unsaturated cobalamin binding proteins in amniotic fluid.

@article{Guant1989ConcentrationAP,
  title={Concentration and physicochemical characterisation of unsaturated cobalamin binding proteins in amniotic fluid.},
  author={J. Gu{\'e}ant and L. Hambaba and M. Vidailhet and C. Schaefer and V. Wahlstedt and J. Nicolas},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1989},
  volume={181 2},
  pages={
          151-61
        }
}
Indirect evidence for the presence of intrinsic factor in amniotic fluid has been provided recently, using either a radioisotope binding assay or a radioimmunoassay. We have determined the unsaturated cobalamin binding capacity and the physicochemical properties of the 3 cobalamin binding proteins in 59 amniotic fluids using radioisotope binding assay, gel filtration and isoelectrofocussing. A good correlation with gestational age was found for the total unsaturated Cbl binding capacity (r = 0… Expand
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Cubilin, the intrinsic factor-vitamin B12 receptor in development and disease.
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