• DOI:10.3892/ijmm.2016.2520
• Corpus ID: 9755050

Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway.

@article{Qin2016ConcentratedGF,
  title={Concentrated growth factor promotes Schwann cell migration partly through the integrin $\beta$1-mediated activation of the focal adhesion kinase pathway.},
  author={Jie Qin and Lin Wang and Ling Zheng and Xiaoyan Zhou and Yidi Zhang and Tingting Yang and Yanmin Zhou},
  journal={International journal of molecular medicine},
  year={2016},
  volume={37 5},
  pages={
          1363-70
        }
}

• Jie Qin, Lin Wang, +4 authors Yanmin Zhou
• Published 1 May 2016
• Biology
• International journal of molecular medicine

Nerve injury is a serious complication associated with dental implant surgery. Following nerve injury, the migration of Schwann cells (SCs) supports nerve regeneration. Concentrated growth factor (CGF) belongs to a new generation of biomaterials that contain fibrin matrix, as well as a number of growth factors that affect the migration of various types of cells, including endothelial cells and cancer cells. To the very best of our knowledge, there are no available studies to date on the promoting effect of CGF on the migration of SCs. Thus, the aim of the present study was to characterize the structure of CGF and growth factor release, examine the effects of CGF on the migration of SCs, and to examine the role of integrin β1 and the focal adhesion kinase (FAK) pathway in the CGF-induced migration of SCs. For this purpose, CGF was prepared by centrifuging rat venous blood, which produced a fiber-like matrix capable of releasing transforming growth factor-β1 (TGF-β1) over a sustained period of time (at least 13 days). The soluble component of CGF was used to produce conditioned media to treat the SC cells in culture. The results demonstrated that CGF promoted the migration of SCs and increased the expression of integrin β1. These effects appeared to involve FAK phosphorylation, which occurred downstream of integrin β1 activation. The short-interfering RNA (siRNA)-mediated downregulation of integrin β1 expression did not block the ability of CGF to promote the migration of SCs. These data suggest that CGF promotes the migration of SCs partly through the integrin β1-mediated activation of the FAK pathway.