Concanamycin A, the specific inhibitor of V-ATPases, binds to the V(o) subunit c.

@article{Huss2002ConcanamycinAT,
  title={Concanamycin A, the specific inhibitor of V-ATPases, binds to the V(o) subunit c.},
  author={Markus Huss and Gudrun Ingenhorst and Simone K{\"o}nig and Michael Gassel and Stefan Dr{\"o}se and Axel Zeeck and Karlheinz Altendorf and Helmut Wieczorek},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 43},
  pages={40544-8}
}
Vacuolar-type ATPase (V-ATPase) purified from the midgut of the tobacco hornworm Manduca sexta is inhibited 50% by 10 nm of the plecomacrolide concanamycin A, the specific inhibitor of V-ATPases. To determine the binding site(s) of that antibiotic in the enzyme complex, labeling with the semisynthetic 9-O-[p-(trifluoroethyldiazirinyl)-benzoyl]-21,23-dideoxy-23-[(125)I]iodo-concanolide A (J-concanolide A) was performed, which still inhibits the V-ATPase 50% at a concentration of 15-20 microm… CONTINUE READING
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