Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs

@inproceedings{Ashworth2010ComputationalRO,
  title={Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs},
  author={Justin Ashworth and Gregory K. Taylor and James J Havranek and S. Arshiya Quadri and Barry L. Stoddard and David P. Baker},
  booktitle={Nucleic acids research},
  year={2010}
}
Site-specific homing endonucleases are capable of inducing gene conversion via homologous recombination. Reprogramming their cleavage specificities allows the targeting of specific biological sites for gene correction or conversion. We used computational protein design to alter the cleavage specificity of I-MsoI for three contiguous base pair substitutions, resulting in an endonuclease whose activity and specificity for its new site rival that of wild-type I-MsoI for the original site… CONTINUE READING
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Heritable targeted mutagenesis in maize using a designed endonuclease.

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