Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants.

@article{Xu2006ComputationalEA,
  title={Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants.},
  author={Ping Xu and Yuanji Zhang and Li Kang and Marilyn J Roossinck and Kirankumar S Mysore},
  journal={Plant physiology},
  year={2006},
  volume={142 2},
  pages={429-40}
}
Successful application of posttranscriptional gene silencing (PTGS) for gene function study in both plants and animals depends on high target specificity and silencing efficiency. By computational analysis with genome and/or transcriptome sequences of 25 plant species, we predicted that about 50% to 70% of gene transcripts in plants have potential off-targets when used for PTGS that could obscure experimental results. We have developed a publicly available Web-based computational tool called… CONTINUE READING