Computational analysis of the number, area and density of gamma-H2AX foci in breast cancer cells exposed to (111)In-DTPA-hEGF or gamma-rays using Image-J software.

@article{Cai2009ComputationalAO,
  title={Computational analysis of the number, area and density of gamma-H2AX foci in breast cancer cells exposed to (111)In-DTPA-hEGF or gamma-rays using Image-J software.},
  author={Zhongli Cai and Katherine Anne Vallis and Raymond M Reilly},
  journal={International journal of radiation biology},
  year={2009},
  volume={85 3},
  pages={262-71}
}
PURPOSE To develop a simple method for the quantification of gamma-H2AX focus number, density and size. METHODS MDA-MB-468 human breast cancer cells were treated overnight with (111)In-diethylenetriaminepentaacetic acid human epidermal growth factor ((111)In-DTPA-hEGF, 0-142 kBq/pmol) or exposed to gamma-radiation to induce DNA double strand breaks (DSB). DNA DSB formation was evaluated by detection of phosphorylated histone H2AX on serine 139 (gamma-H2AX) using immunofluorescence. Confocal… CONTINUE READING
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