Computational Design of Stable and Soluble Biocatalysts

@article{Musil2019ComputationalDO,
  title={Computational Design of Stable and Soluble Biocatalysts},
  author={Milos Musil and Hannes Konegger and Jir{\'i} Hon and David Bednar and Jiř{\'i} Damborsk{\'y}},
  journal={ACS Catalysis},
  year={2019},
  volume={9},
  pages={1033-1054}
}
Natural enzymes are delicate biomolecules possessing only marginal thermodynamic stability. Poorly stable, misfolded, and aggregated proteins lead to huge economic losses in the biotechnology and biopharmaceutical industries. Consequently, there is a need to design optimized protein sequences that maximize stability, solubility, and activity over a wide range of temperatures and pH values in buffers of different composition and in the presence of organic cosolvents. This has created great… Expand

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