Computation vs. cloning: evaluation of two methods for haplotype determination.

@article{Harrigan2008ComputationVC,
  title={Computation vs. cloning: evaluation of two methods for haplotype determination.},
  author={Ryan J Harrigan and Maureen E. Mazza and Michael D Sorenson},
  journal={Molecular ecology resources},
  year={2008},
  volume={8 6},
  pages={1239-48}
}
Nuclear sequence data, often from multiple loci, are increasingly being employed in analyses of population structure and history, yet there has been relatively little evaluation of methods for accurately and efficiently separating the alleles or haplotypes in heterozygous individuals. We compared the performance of a computational method of haplotype reconstruction and standard cloning methods using a highly variable intron (ornithine decarboxylase, intron 6) in three closely related species of… CONTINUE READING