Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation

  title={Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation},
  author={Michele M. Pascucci and Shankar Ganesan and Ashok Kumar Tripathi and Ori Katz and Valentina Emiliani and Marc Guillon},
  journal={Nature Communications},
  • Michele M. Pascucci, Shankar Ganesan, +3 authors Marc Guillon
  • Published 2019
  • Biology, Physics, Medicine, Materials Science
  • Nature Communications
  • Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination patterns are combined with the saturation of an optical transition to enable sub-diffraction imaging. While the technique proved useful for two-dimensional imaging, extending it to three-dimensions is challenging due to the fading of organic fluorophores under… CONTINUE READING

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    Publications referenced by this paper.

    Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution.

    • Mats G L Gustafsson
    • Chemistry, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2005

    Saturated patterned excitation microscopy--a concept for optical resolution improvement.

    Compressive fluorescence microscopy for biological and hyperspectral imaging