Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation

@article{Pascucci2019CompressiveTS,
  title={Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation},
  author={Michele M. Pascucci and Shankar Ganesan and Ashok Kumar Tripathi and Ori Katz and Valentina Emiliani and Marc Guillon},
  journal={Nature Communications},
  year={2019},
  volume={10}
}
  • Michele M. Pascucci, Shankar Ganesan, +3 authors Marc Guillon
  • Published 2019
  • Biology, Physics, Medicine, Materials Science
  • Nature Communications
  • Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination patterns are combined with the saturation of an optical transition to enable sub-diffraction imaging. While the technique proved useful for two-dimensional imaging, extending it to three-dimensions is challenging due to the fading of organic fluorophores under… CONTINUE READING

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