Compression loading in vitro regulates proteoglycan synthesis by tendon fibrocartilage.


The regulation of proteoglycan synthesis in a fibrocartilaginous tissue by mechanical loading was assessed in vitro. Discs of bovine tendon fibrocartilage were loaded daily with unconfined, cyclic, uniaxial compression (5 s/min, 20 min/day) and the synthesis of large and small proteoglycans was measured by incorporation of [35S]sulfate. All discs synthesized predominantly large proteoglycan when first placed in culture. After 2 weeks in culture nonloaded discs synthesized predominantly small proteoglycans whereas loaded discs continued to produce predominantly large proteoglycan. The turnover of 35S-labeled proteoglycan was not significantly altered by the compression regime. Increased synthesis of large proteoglycans was induced by a 4-day compression regime following 21 days of culture without compression. Inclusion of cytochalasin B during compression mimicked this induction. Autoradiography demonstrated that cell proliferation was minimal and confined to the disc edges whereas 35S-labeled proteoglycan synthesis occurred throughout the discs. These experiments demonstrate that mechanical compression can regulate synthesis of distinct proteoglycan types in fibrocartilage.

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@article{Koob1992CompressionLI, title={Compression loading in vitro regulates proteoglycan synthesis by tendon fibrocartilage.}, author={Thomas J. Koob and Pamela E. Clark and David J. Hernandez and Frederick A. Thurmond and Kathryn G. Vogel}, journal={Archives of biochemistry and biophysics}, year={1992}, volume={298 1}, pages={303-12} }