Compression loading in vitro regulates proteoglycan synthesis by tendon fibrocartilage.

Abstract

The regulation of proteoglycan synthesis in a fibrocartilaginous tissue by mechanical loading was assessed in vitro. Discs of bovine tendon fibrocartilage were loaded daily with unconfined, cyclic, uniaxial compression (5 s/min, 20 min/day) and the synthesis of large and small proteoglycans was measured by incorporation of [35S]sulfate. All discs synthesized predominantly large proteoglycan when first placed in culture. After 2 weeks in culture nonloaded discs synthesized predominantly small proteoglycans whereas loaded discs continued to produce predominantly large proteoglycan. The turnover of 35S-labeled proteoglycan was not significantly altered by the compression regime. Increased synthesis of large proteoglycans was induced by a 4-day compression regime following 21 days of culture without compression. Inclusion of cytochalasin B during compression mimicked this induction. Autoradiography demonstrated that cell proliferation was minimal and confined to the disc edges whereas 35S-labeled proteoglycan synthesis occurred throughout the discs. These experiments demonstrate that mechanical compression can regulate synthesis of distinct proteoglycan types in fibrocartilage.

050100150'98'00'02'04'06'08'10'12'14'16
Citations per Year

591 Citations

Semantic Scholar estimates that this publication has 591 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Koob1992CompressionLI, title={Compression loading in vitro regulates proteoglycan synthesis by tendon fibrocartilage.}, author={Thomas J. Koob and Pamela E. Clark and David J. Hernandez and Frederick A. Thurmond and Kathryn G. Vogel}, journal={Archives of biochemistry and biophysics}, year={1992}, volume={298 1}, pages={303-12} }