Compression and crevasses in vitreous sections under different cutting conditions.

@article{Han2008CompressionAC,
  title={Compression and crevasses in vitreous sections under different cutting conditions.},
  author={H-M Han and Beno{\^i}t Zuber and Jacques Dubochet},
  journal={Journal of microscopy},
  year={2008},
  volume={230 Pt 2},
  pages={
          167-71
        }
}
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent… CONTINUE READING
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SHOWING 1-7 OF 7 REFERENCES

Characterization of the cutting edge of glass and diamond knives for ultramicrotomy by scanning force microscopy using cantilevers with a defined tip geometry

  • T. R. Matzelle, H. Gnaegi, A. Ricker, R. Reichelt
  • Part II. J. Microsc
  • 2003
1 Excerpt

Electron scattering in ice and organic materials

  • R. Eusemann, H. Rose, J. Dubochet
  • J. Microsc
  • 1982
2 Excerpts

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